By Rodolfo Paoletti; David Kritchevsky
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They are then embedded in Arnaldite Teflon molds, thin sectioned, and stained with uranyl acetate and lead citrate (Jones, 1976). T o evaluate the intimal thickness, distribution and involvement of various cell types as well as the a m o u n t of fiber protein present, sections 2-3 /mi thick are also prepared. 2) for 1 minute, rinsed, and coverslipped (Jones, 1976). T o demonstrate apo-B localization Hoff and G a u b a t z (1975) followed a fixation method which began with washes in cacodylate buffer, followed by incubation of thin sections in a peroxidase localization medium and hydrogen peroxide.
This method has been used widely and successfully to demonstrate the presence of fibrin and fibrinogen in early and late lesions (Willey et al, 1964; Woolf and Carstairs, 1967) as well as platelet material ( H u d s o n and McCaughey, 1974). Several groups of investigators have used this immunochemical ap proach in an attempt to identify the lipoprotein(s) present in a t h e r o m a t o u s lesions and identified a protein whose immunochemical properties are similar to those of the apo-protein of L D L (Tracy et al, 1961; Watts, 1963; Pathology in Atherosclerosis Research 41 Kao and Wissler, 1965; Woolf and Pilkington, 1965; Wissler and Vessel inovitch, 1969; Walton and Williamson, 1968; A d a m s , 1971).
Prospective epidemiological studies use two different approaches: One is to study individuals in a defined population using all the tools of modern clinical diagnosis in order to formulate a detailed profile of that particular individual. These clinical findings are then related t o the development of the disease in that particular case. Such studies are referred to as clinical epidemiological studies. The second approach involves large populations and usually employs rather crude methods of assessment of both the disease process and the population characteristics.