By Rafinesque C.S
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The magnification, given by the ratio between the focal lengths of L5 and L3, is 15× to provide a 390 μm × 390 μm field of view on the CCD sensor. According to , the maximum refocusing distance, in the coherent case, without significant loss of resolution, is approximately ±250 μm around the best focus plane. 42 F. Dubois et al. a b Fig. 1 Biomedical Applications The biomedical applications that we present below have been performed with the DHM illumination with a LED, as described in Fig. 1.
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