Analysis of Samples of Clinical and Alimentary Interest with by Emilia Witkowska Nery

By Emilia Witkowska Nery

This publication provides major units of paper-based analytical structures. the 1st set is a platform for the research of glucose, ldl cholesterol and uric acid in organic samples, and the second one set is a state-of-the-art digital tongue process for the research of drinks (mineral water, beer, wine). This thesis additionally presents an in depth overview of 33 tools of enzyme immobilization on paper which were evaluated to augment the garage balance of the proposed approach for biomarker detection. From a pragmatic standpoint, this thesis covers a various set of subject matters concerning paper-based sensing, together with colorimetric and electrochemical detection equipment, diversified units of structure (spot-tests, lateral and tangential circulation assays), tools of fabrication (wax printing, slicing, impregnation with polymers), measurements in desk bound and circulate stipulations to boot machine modeling of proposed structures and complicated information research utilizing chemometric thoughts. This publication turns out to be useful for PhD scholars operating during this or a comparable box who require precise information regarding technique and historical past to this learn.

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Depending on the color and hue of the image analyzed, the total value or just one channel of the color space can be used for quantification. Usually the quantitative response is given as the arithmetic mean of the pixel intensity within the detection zone after subtraction of the mean intensity for the control region. As it was already mentioned, optical detection presents many assets nevertheless some variables must be taken into consideration, for example the fact that differences in illumination will cause variations in intensity and hue of a given color.

Nery EW, Kubota LT (2013) Sensing approaches on paper-based devices: a review. Anal Bioanal Chem 405:7573–7595 16. Jagadeesan KK, Kumar S, Sumana G (2012) Application of conducting paper for selective detection of troponin. Electrochem Commun 20:71–74. 041 17. Tseng S-C, Yu C-C, Wan D et al (2012) Eco-friendly plasmonic sensors: using the photothermal effect to prepare metal nanoparticle-containing test papers for highly sensitive colorimetric detection. Anal Chem 84:5140–5145. 1021/ac300397h 18.

EG, Johnston DG (2009) Glucose sensors: A review of current and emerging technology. Diabet Med 26:197–210. 2008. x References 21 93. Allen MP, DeLizza A, Ramel U et al (1990) A noninstrumented quantitative test system and its application for determining cholesterol concentration in whole blood. Clin Chem 36:1591–1597 94. Allen MP (1995) Laminated assay device. 15:1–15 95. Martinez AW, Phillips ST, Butte MJ, Whitesides GM (2007) Patterned paper as a platform for inexpensive, low-volume, portable bioassays.

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