By Shaw A M
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Additional info for Astrochemistry[c] From Astronomy to Astrobiology
1 and 3). , 1968a, 1968 b), two events are required for the production of DNAless bacteria. One is the formation of filaments with extremities free of nuclei (obtained for instance by the mutation listed above), the other is the Div mutation which causes the filaments to divide thereafter. 1. The expression of the thermosensitive defect The cessation of either DNA synthesis or partition of sister DNA's at the non-permissive temperature, is somehow involved in the production of DNA-less bacteria.
Although evidence exists that at least some plasmids segregate with the chromosome in an orderly manner, others may be free in the cytoplasm or linked to the membrane at sites other than that to which the chromosome is presumed to be attached. In the last few years several groups of workers have made use of the minicell-producing strains for the isolation and characterization of plasmida For example, Kass and Yarmolinsky (1970) established that the sex factor Ffgal and F f X could be isolated from minicells formed during the growth of strains harboring these plasmids.
E. DNA-chain elongation mutants which stop DNA synthesis immediately after the temperature shift also show an hour lag. The number of nuclei per cell is reduced during the time of cell division : thereafter the septation of cells is inhibited, and filamentous cells are formed. In the filamentous cells, the nuclear mass remains at the central region leaving both cell-termini without DNA, as is also the case when DNA synthesis is stopped by thymine starvation (Cohen and Barner, 1954). In contrast to thymine starvation, during which cell division is completely stopped, many thermosensitive mutants of DNA synthesis continue residual septations for several generations at the reduced rate.