Board Review Series: Biochemistry by Dawn B. Marks

By Dawn B. Marks

Temple Univ. tuition of medication, Philadelphia, PA. assessment in define layout for clinical scholars getting ready for USMLE Step 1 or direction assessments. earlier variation: c1994. Softcover.

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Acquisition times in the indirect dimension(s) of 2-, 3- and 4- dimensional experiments are set via the number of acquisitions, or increments, and are in turn related to the spectral widths in these dimensions. The relationship of AQ ¼ N/(2SW) (where AQ is the total acquisition time, N is the number of increments and SW the spectral width) is well known. 26/R2, where R2 is the transverse relaxation rate [16,19], but this is rarely possible to achieve, and so a balance must be struck. This balance is additionally complicated by the constraints of the phase cycle of the NMR experiment itself.

2 Additives As many NMR experiments require hours or days to complete, addition of anti-microbial agents is highly recommended. g. some cytochromes) micromolar concentrations of an antibiotic such as ampicillin or chloramphenicol can be substituted. 1–5 mM in order to reduce proteolysis. However, these compounds have nonexchangeable protons that can interfere with the spectrum of the sample. Excessive use of these compounds is best avoided, a better approach being to improve the purification protocol.

NMR, 25, 349–354. Sample Preparation, Data Collection and Processing 21 26. Rovnyak, D. et al. (2004) Accelerated acquisition of high resolution triple-resonance spectra using non-uniform sampling and maximum entropy reconstruction. J. Magn. , 170, 15–21. 27. Marion, D. (2005) Fast acquisition of NMR spectra using Fourier transform of non-equispaced data. J. Biomol. NMR, 32, 141–150. 28. , Kupce, E. and Brutscher, B. (2005) SOFAST-HMQC experiments for recording twodimensional heteronuclear correlation spectra of proteins within a few seconds.

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