Carbohydrate Antigens by Per J. Garegg and Alf A. Lindberg (Eds.)

By Per J. Garegg and Alf A. Lindberg (Eds.)

content material: Michael Heidelberger : April 29, 1888-June 25, 1991 / Elvin A. Kabat --
How proteins realize and bind oligosaccharides / R.U. Lemieux --
Synthesis and immunological houses of glycopeptide T-cell determinants / Morten Meldal, Søren Mouritsen, and Klaus Bock --
Human fucosyltransferases taken with the biosynthesis of X (Gal-[beta]-1-4[Fuc-[alpha]-1-3]GlcNAc) and sialyl-X (NeuAC-[alpha]-2-3Gal-[beta]-1-4[Fuc-[alpha]-1-3]GlcNAc) antigenic determinants / Winifred M. Watkins, Patricia O. Skacel, and Philip H. Johnson --
Vibrio cholerae polysaccharide stories / Bengt Lindberg --
Distinguishing antibodies that bind inner antigenic determinants from those who bind the antigenic chain-terminus merely / Cornelis P.J. Glaudemans, Larry G. Bennett, Apurba okay. Bhattacharjee, Eugenia M. Nashed, and Thomas Ziegler --
Purification of oligosaccharide antigens through vulnerable affinity chromatography / D.A. Zopf and W-T. Wang --
Synthesis and conformational research of the Forssman pentasaccharide and di-, tri-, and tetrasaccharide fragments / G. Magnusson, U. Nilsson, A.K. Ray, and K.G. Taylor --
Synthesis and immunochemistry of carbohydrate antigens of the [beta]-hemolytic streptococcus crew A / B. Mario Pinto --
Synthesis of antigenic heptose- and Kdo- containing oligosaccharides of the interior center zone of lipopolysaccharides / H. Paulsen, E.C. Höffgen, and M. Brenken --
Molecular biology of anti-[alpha]-(1 [yields] 6)dextrans : antibody responses to a single-site-filling antigenic determinant / Elvin A. Kabat --
prolonged kind 1 chain glycosphingolipids : Lea-Lea (dimeric Lea) and Leb-Lea as human tumor linked antigens / Mark R. Stroud, Steven B. Levery, and Sen-itiroh Habomori.

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REFERENCES 1. Harding C. ; Roof, R. ; Allen, P. R. Proc. Natl. Acad. Sci. USA 1991, 88, 2740. 2. ; Ling, N. ; Guillemin, R. C. Carbohydr. Res. 1981, 89, 221. 3. ; B. Dombo, B. Angew. , Int. Ed. Engl. 1988, 27, 711. 4. ; Weichert, U. Angew. , Int. Ed. Engl. 1988, 27, 1365. 5. ; Tejbrant, J. J. Chem. , Chem. Commun. 1989, 1267. ch003 3. MELDALETAL. Glyeopeptide T-Cell Determinants 33 6. ; Foffani, M. ; Rocchi, R. Int. J. Biol. Macromol. 1990, 12, 41. 7. ; Torres, J. ; Valencia G. Angew. , Int. Ed.

Methoxide solution (IN) was prepared from sodium and dry methanol and diluted prior to use. HPLC was carried out on a Waters Delta Prep 3000 HPLC system with a E600 pump, a 991 photodiode array detector and preparative and analytical radial pack RP-C columns. 1% TFA in 90 % aqueous acetonitrile. Medium pressure chromatography was carried out on dry packed silica gel 60 from Merck with appropriate mixtures of ethyl acetate and petroleum ether. Silica coated aluminum TLC plates were eluted with mixtures of ethyl acetate and petroleum ether and developed by charring with sulfuric acid spray.

J. Chem. Soc. Perkin Trans. 1. 1988, 2895. 30. ; Duus, J. ; Pedersen, S. Carbohydr. ,1991, 211, 219. 31. ; Holm, A. Scand. J. Immunol. 1988, 28, 261. RECEIVED April 15, 1992 Chapter 4 Human Fucosyltransferases Involved in the Biosynthesis of X (Gal-β-1-4[Fuc-α1-3]GlcNAc) and Sialyl-X (NeuAC-α-2-3Gal-β-1-4[Fuc-α1-3]GlcNAc) Antigenic Determinants 1 2 3 Winifred M. Watkins , Patricia O. Skacel , and Philip H. ch004 1 Department of Haematology, Royal Postgraduate Medical School, Hammersmith Hospital, London W12 ONN, England Department of Haematology, Northwick Park Hospital, Watford Road, Harrow, Middlesex HA1 3UJ, England Medical Research Council Human Biochemical Genetics Unit, University College, London NW1 2HE, England 2 3 The X structure (Gal-β-1-4[Fuc-α-1-3]GlcNAc) occurs in glycoproteins, glycolipids, and free oligosaccharides and may be exposed as a terminal non-reducing end-group or masked by substitution with other sugars.

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