By Q. Ivy Fan, Kathleen M. Vanderpool, Jessica O’Connor (auth.), Elissavet Kardami, Larry Hryshko, Nasrin Mesaeli (eds.)
Cardiac cellphone biology has come of age.
reputation of activated or changed signaling molecules by way of particular antibodies, new selective inhibitors, and fluorescent fusion tags are yet some of the instruments used to dissect signaling pathways and cross-talk mechanisms that could ultimately enable rational drug layout. realizing the law of cardiac hypertrophy in all its complexity is still a primary target of cardiac examine. because the development of adenovirally mediated gene move, transfection potency isn't any longer a restricting think about the learn of cardiomyocytes. A restricting think about contemplating mobilephone transplantion as a technique to fix the broken center is telephone availability on the correct time. Cardiac hole junctions, intercellular verbal exchange channels that permit electric and metabolic coupling and play a huge function in arrhythmogenesis at the moment are understood to be beautiful sensors of cardiac swap.
The experiences during this quantity comprise dependent reports that made use of leading edge technological advances and lots of really expert reagents to deal with those issues.
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Extra info for Cardiac Cell Biology
3. Effect ofWT-Cx43 or Cx43-CT on HeLa cell proliferation. HeLa cells stably expressing WT - or CT -Cx43 (as indicated) were plated at 20,000 cell per well (24-well plates) and maintained in the presence of I % serum. D. (n = 4). 05 was considered significant. concluded that the 'free' C-tail ofCx43 can have significant biological effects on cells such as inhibition of proliferation, and that these effects do not require plasma membrane-dependent, channel-forming functions. The mechanism of growth inhibition by CT-Cx43 remains to be established.
I:"' « ~ Ul ~ '"> ;z. + IOOAI o « W :I: ~ ~ f + SIOOAI B. B. ;:; h~ ,.... I'l ~ '" "' If:~ ~ , a. + SIOOA 1 c. , dZ i:~~ ffi ~ Cl. :> + SIOOAI Fig. 2. SIOOAI inhibits transcnptlOn from the human SIOOB promoter through elements located upstream of the minimal promoter. Neonatal rat cardiac myocytes were co-transfected with RSV-CAT and pGBS -162/+698 (minimal SIOOB promoter) (A) or pGBS --6698/ +698 (maximal SIOOB promoter) (B) or co-transfected with the above and S I OOA I as indicated. Myocytes treated with vehicle were co-transfeeted with the plasmid vector.
Non-transfected HeLa cells or myocytes displayed no immunoreactive bands or only the -43--45 kDa intact Cx43 bands, respectively (data not shown). 5 Cyt Fig. 1. Expression and localization of Cx43 constructs in cardiomyocytes and HeLa cells, detected by immunofluorescence. (A and B) Cardiomyocyte cultures transfected with cDNAs expressing NT -Cx43 (FLAG-tagged) or CT-Cx43, respectively and stained for FLAG (A) or Cx43 (B). (C and D) HeLa cells transfected with cDNAs expressing WT -Cx43 or CT -Cx43, respectively, and stained with anti-Cx43 antibodies.