Cell Separation: Fundamentals, Analytical and Preparative by Maria B. Dainiak, Ashok Kumar, Igor Yu. Galaev, Bo

By Maria B. Dainiak, Ashok Kumar, Igor Yu. Galaev, Bo Mattiasson (auth.), Ashok Kumar, Igor Yu Galaev, Bo Mattiasson (eds.)

M. B. Dainiak, A. Kumar, I.Y. Galaev, B. Mattiasson: equipment in mobilephone Separations.-

S.F. Ibrahim, G. van den Engh: circulate Cytometry and telephone Sorting.-

A.A. Neurauter, M. Bonyhadi, E. Lien, L. Nøkleby, E. Ruud, S. Camacho, T. Aarvak: telephone Isolation and enlargement utilizing Dynabeads®.-

J. Hubble: Affinity Adsorption of Cells to Surfaces and techniques for phone Detachment.-

M.B. Dainiak, I.Y. Galaev, A. Kumar, F.M. Plieva, B. Mattiasson: Chromatography of dwelling Cells utilizing Supermacroporous Hydrogels, Cryogels.-

R.E. Nordon, S. Craig: Hollow-Fibre Affinity telephone Separation.-

J.M.S. Cabral: mobile Partitioning in Aqueous Two-Phase Polymer Systems.-

M. Kamihira, A. Kumar: improvement of Separation method for Stem Cells.-

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In this regard, high-speed flow cytometers may make the most impact, as these applications demand the greatest need for higher throughput and flexibility in measurement parameters; cells to be infused into a patient should be extracorporeal for the shortest time possible and isolated to the utmost level of purity. For instance, in the case of bone marrow transplantation, it was unknown which of the transplanted cells had the ma- Flow Cytometry and Cell Sorting 29 jor regenerative capacity. As a result, patients would receive transplants of whole HLA-identical sibling bone marrow and hope for the best outcome.

67. 68. 69. 70. 71. 72. 73. 74. 75. 76. 77. 78. 79. 80. 81. F. Ibrahim · G. van den Engh Drosophila follicle cells: toward a general strategy for cell type-specific developmental analysis. Proc Natl Acad Sci USA 96:5559–5564 Li J, Greco V, Guasch G, Fuchs E, Mombaerts P (2007) Mice cloned from skin cells. Proc Natl Acad Sci USA 104(8):2738–2743 Ibrahim SF, Diercks AH, Petersen TW, van den Engh G (2007) Kinetic analyses as a critical parameter in defining the side population (SP) phenotype. Exp Cell Res 313(9):1921–1926 Petersen TW, Ibrahim SF, Diercks AH, van den Engh G (2004) Chromatic shifts in the fluorescence emitted by murine thymocytes stained with Hoechst 33342.

Much of the early work outlining hematopoiesis was achieved in this fashion and eventually contributed to the first isolations of the hematopoietic stem cell [59]. Currently, cell sorters continue to be at the crux of stem cell research [60, 61] and cell type-specific developmental analysis [62]. One would be hard-pressed to find a many publications relating to stem cells that did not employ flow cytometry and cell sorting as part of their methods [49, 61]. In a recent report, epidermal skin stem cells were isolated in a flow cytometer based on surface markers, and their nuclei were reprogrammed to grow mice by their exposure to the cytoplasm of unfertilized murine oocytes [63].

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