By Chang Lu
Chapter 1 starting place, present prestige, and destiny views of Chemical Cytometry (pages 1–19): Norman J. Dovichi
Chapter 2 Metabolic Cytometry – The learn of Glycosphingolipid Metabolism in unmarried basic Cells of the Dorsal Root Ganglia (pages 21–30): Colin D. Whitmore, Jillian Prendergast, David Essaka, Ole Hindgaul, Monica P. Palcic, Ronald L. Schnaar and Norman J. Dovichi
Chapter three cellphone Signaling Studied on the Single?Cell point (pages 31–54): Angela Proctor, Shan Yang, Sumith Kottegoda, Michael Brenner, Ryan M. Phillips, Christopher E. Sims and Nancy L. Allbritton
Chapter four Ultrasensitive Detection of Low?Copy?Number Molecules from unmarried Cells (pages 55–73): Kangning Ren and Hongkai Wu
Chapter five Capillary Electrophoresis of Nucleic Acids on the Single?Cell point (pages 75–91): Ni Li and Wenwan Zhong
Chapter 6 Microfluidic know-how for Single?Cell research (pages 93–106): Yan Chen and Jiang F. Zhong
Chapter 7 On?Chip Electroporation and Electrofusion for Single?Cell Engineering (pages 107–124): Ana Valero and Albert van den Berg
Chapter eight Electroporative movement Cytometry for Single?Cell research (pages 125–142): Prof. Chang Lu, Jun Wang, Ning Bao and Hsiang?Yu Wang
Chapter nine Ultrasensitive research of person Cells through Droplet Microfluidics (pages 143–157): Robert M. Lorenz and Daniel T. Chiu
Chapter 10 Probing Exocytosis at unmarried Cells utilizing Electrochemistry (pages 159–174): Yan Dong, Michael L. Heien, Michael E. Kurczy and Andrew G. Ewing
Chapter eleven Electrochemical decision of Enzyme task in unmarried Cells (pages 175–195): Wenrui Jin
Chapter 12 Single?Cell Mass Spectrometry (pages 197–218): Ann M. Knolhoff, Stanislaw S. Rubakhin and Jonathan V. Sweedler
Chapter thirteen Optical Sensing Arrays for Single?Cell research (pages 219–237): Ragnhild D. Whitaker and David R. Walt
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Additional info for Chemical Cytometry: Ultrasensitive Analysis of Single Cells
Neuhoff, V. (2000) Microelectrophoresis and auxiliary micromethods. Electrophoresis, 21, 3–11. J. J. (1968) Electrophoretic analysis of antibody produced by single cells. Proc. Natl. Acad. Sci. , 61, 860–867. L. (1971) Molecular weight distribution of proteins synthesized in References 18 19 20 21 22 23 24 25 26 27 single, identiﬁed neurons of Aplysia. J. Gen. , 57, 26–40. Ruchel, R. (1976) Sequential protein analysis from single identiﬁed neurons of Aplysia californica. A microelectrophoretic technique involving polyacrylamide gradient gels and isoelectric focusing.
When choosing a reporter, there are several parameters that must be examined. First of all, the reporter must be able to be loaded into the cell. 2 Analytes Examined and Reporters Used achieved by altering the electrophoretic mobility of the reporter. The chosen reporter must be distinguishable from cellular components and must be compatible with the chosen detection method. Fluorescently labeled substrates are often used as reporters because of the very low detection limits afforded by LIF detection.
19 21 2 Metabolic Cytometry – The Study of Glycosphingolipid Metabolism in Single Primary Cells of the Dorsal Root Ganglia Colin D. Whitmore, Jillian Prendergast, David Essaka, Ole Hindgaul, Monica P. Palcic, Ronald L. Schnaar, and Norman J. 1 Glycosphingolipids Play an Important Role in Neuronal Membranes The surfaces of animal cells are covered with glycoproteins and glycolipids. The sugar chains, termed oligosaccharides, face the outside of the cells where they can act as recognition molecules in a very wide array of biological events [1–3].