Current research in embryology by Sabine Globig

By Sabine Globig

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Am J Physiol 1997, 272(1 Pt 1):E173–180. 13. Reece EA, Wu YK, Zhao Z, Dhanasekaran D: Dietary vitamin and lipid therapy rescues aberrant signaling and apoptosis and prevents hyperglycemia-induced diabetic embryopathy in rats. Am J Obstet Gynecol 2006, 194(2):580–585. Maternal Diabetes Alters Transcriptional Programs 43 14. Li R, Chase M, Jung SK, Smith PJ, Loeken MR: Hypoxic stress in diabetic pregnancy contributes to impaired embryo gene expression and defective development by inducing oxidative stress.

The pregnant rat uteri were injected with either DD water, or Hsp105 S-ODNs or Hsp105 A-ODNs on day 3 of pregnancy, the uteri were collected 24 h and 48 h later, and then subjected to immunostaining analysis. As shown in Fig. 6A (a, b), an intensive staining was observed mainly in the luminal epithelium and glandular epithelial cells in the uterus treated with water and S-ODNs respectively. In contrast, the contralateral horn treated with A-ODNs showed only low level of Hsp105 staining on day 4, 24h after injection of ODNs (Fig.

The sections were incubated with the primary antibodies against Hsp105 (sc-6241, Santa Cruz Biotechnology, Santa Cruz, CA 1: 200) in 10% goat serum overnight at 4°C. The sections were then washed three times with PBS (10 min each) and incubated with biotin labeled secondary antibody (goat anti-rabbit IgG, RT, 30 min, 1:200), After three times washes with PBS, the sections were incubated with avidin-AP complex (1:200, RT, 20 min). After three more washes, the sections were developed with Vector Red AP substrates according to the manufacturer's protocol (Vectastain ABCAP kit, Vector Laboratories, Burlingame, CA).

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