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Extra info for Dynamic Analysis of a Multi-Mesh Helical Gear Train
Releasing the block. 5-8 h to obtain good results. 5-5 h. 3. Add 100 p,L of colcemid for the final 15-30 min to arrest the cells in metaphase (see Note 1). 2. Ethidium Bromtde Technique (Originally Described by Ikeuchi [2U. 1. When mamtammg cells for making chromosomes by this method, the cells are kept m a semiconfluent state and only split when the flask is fully confluent (stationary phase). This will give a very crude but reasonably effective means of synchronizing rapidly growing cells. However, the chromosomes are in a more nearly “native” state after this.
8. Rinse surplus dye solution off with distilled water, and carefully blot the slides dry. 9. , DPX). Do not use Canada balsam, which is acidic and causes fading. A C-banded mammalian metaphase spread is illustrated in Fig. ). 2. Gll Banding Gl 1 banding involves, essentially, staining chromosome preparations with an alkaline solution of Giemsa. This produces distinctive staining of a subset of heterochromatic bands in humans and the great apes. Characteristically, the paracentric heterochromatin of human chromosome 9 is stained a magenta color, whereas the chromosome arms are blue.
Cytogenet. Cell Genet. , McBeath, S , and Hargreave, T. B. (1986) A human 9;20 reciprocal translocatron associated with male infertility, analysed at prophase and metaphase I of meiosis Cytogenet Cell Genet 41, 145-153 CHAPTER4 Preparation of Chromosomes for Scanning Electron Microscopy Adrian l! Sumner, Andrew R. Ross, and EZizabeth Graham 1. Introduction Use of scanning electron microscopy (SEM) to study chromosomes permits their observation at higher resolution than is possible by light microscopy and,at the sametime, provides aesthetically pleasing images.