Electroporation and Sonoporation in Developmental Biology by Harukazu Nakamura Phd (auth.), Harukazu Nakamura Phd (eds.)

By Harukazu Nakamura Phd (auth.), Harukazu Nakamura Phd (eds.)

In ovo electroporation is an epoch-making fulfillment within the research of developmental biology. With this technique, experiments will be performed in achieve and lack of functionality in wanted tissue at any wanted level in chick embryos. advent of a tetracycline-regulated gene expression method and a transposon method has additional prolonged the potential for the strategy, making it attainable to acquire long term expression and to show off and on a gene of curiosity. it's now utilized to mice, aquatic animals, or even to crops for the learn of developmental biology and for different reasons. during this e-book, the applying of electroporation in lots of embryonic tissues and organs is brought, with a few chapters that care for gene move in adults. Sonoporation, one other useful gizmo, utilizing ultrasonic waves rather than electrical currents, for gene move to mesenchymal tissues is additionally brought.

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Gene transfer into cultured mammalian embryos by electroporation. Methods 24, 35–42. , Nakatsuji, N. (2001). Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Dev Biol 240, 237–246. , Takahashi, Y. (2007). Stable integration and conditional expression of electroporated transgenes in chicken embryos. Dev Biol 305, 616–624. , Nakatsuji, N. (1990). A mouse embryonic stem cell line showing pluripotency of differentiation in early embryos and ubiquitous beta-galactosidase expression.

2000). Misexpression of genes in brain vesicles by in ovo electroporation. Dev Growth Differ 42, 199–201. , Miyazaki, J. (1991). Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108, 193–199. , Takahashi, Y. (2007). Stable integration and conditional expression of electroporated transgenes in chicken embryos. Dev Biol 305, 616–624. , Takahashi, Y. (2008). Notch mediates the segmental specification of angioblasts in somites and their directed migration toward the dorsal aorta in avian embryos.

Dev Biol 305, 625–636. Chapter 2 In Ovo Electroporation as a Useful Tool to Pursue Molecular Mechanisms of Neural Development in Chick Embryos Noritaka Odani, Xubin Hou, and Harukazu Nakamura 1 Introduction In the field of developmental biology, electroporation has become a routine technique for gene transfer because of its simplicity and wide range of application. , 2000). Especially, many gene analyses were conducted in developing neural tube, because the neural tube is a convenient tissue for application of in ovo electroporation.

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