Enzyme purification and related techniques by William B. Jakoby (Eds.)

By William B. Jakoby (Eds.)

The severely acclaimed laboratory commonplace, Methods in Enzymology, is without doubt one of the such a lot hugely revered courses within the box of biochemistry. in view that 1955, every one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless appropriate this present day - actually a vital ebook for researchers in all fields of existence sciences

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Simple gravity flow systems utilizing flow in thin channels, parallel to the membrane surface, are also available; the pressure gradient for ultrafiltration is provided by a reduced pressure in the ultrafiltrate line. Although, in the former system, there is better control of concentration ratios, either system should be effective in terms of minimum denaturation of the retained macrosolutes. There are a large number of manufacturers providing UF systems for general application. Table II primarily includes those companies with equipment more suited to laboratory or pilot plant use as categorized in the preceding section.

Many of the recipes for dialysis that are reported in the literature described the size of tubing employed by the Visking Company's nomen3The techniques described below were all p e r f o r m e d with such tubing, obtained from the Visking Company, a division o f Union Carbide Corporation, 6733 West 65th Street, Chicago, Illinois 60638. [4] DIALYSIS 25 clature. A change in ownership of the company was accompanied by a change in nomenclature. Since this may cause some confusion, it seems worthwhile to explain the two systems.

FIG. 3. The rapid dialyzer of E. Cabib and I. D. Algranati. Reproduced from Nature (London) [ 188, 409 ( 1960)] by courtesy of the authors and publishers. Cabib and I. D. Algranati, Nature (London) 188, 409 (1960). [5] LYOPHILIZATION 33 [5] Lyophilization /33, JOHANNES EVERSE and FRANCIS E. STOLZENBACH Application Lyophilization, the drying of materials in the frozen state, is a technique that is widely used in the laboratory as well as in industrial processes for the concentration, storage, and distribution of biological materials.

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