Green Fluorescent Protein: Applications & Protocols (Methods by Barry W. Hicks (Editor)

By Barry W. Hicks (Editor)

Barry W. Hicks and a crew of well-practiced experimentalists display the ability and the flexibility of the golf green fluorescent protein (GFP) marker with a state of the art number of with ease reproducible GFP strategies. those comprise tools for detecting and imaging GFP (FRET, BRET, and whole-body imaging); for utilizing GFP to create and choose for transgenic organisms, for its use as a biosensor; and for learning viruses. A useful better half CD-ROM monitors many colour figures and movies from over part the chapters.

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Termini of variants generated by random circular permutation are indicated by squares (17), and rationally designed permuted variants are indicated by circles (16). The linker had the sequence (Gly)2Thr-(Gly)2-Ser, in the case of the random approach, and the sequence Gly-Ser-(Gly)2Thr-Gly, in the case of the rational approach. The first and last residues of regular secondary structures, as determined by Yang et al. (28), are indicated. The position of the chromophore in the central helical segment (56–72) is marked by a star.

Acids Res. 23, 1087–1088. 13. Lukyanov, K. , Siebert, P. , Usman, N. , Matz, M. , and Lukyanov, S. A. 18 of total RNA using the suppression PCR effect. Biophys. Biochem. Res. Comm. 230, 285–288. 14. Schmidt, W. M. and Mueller, M. W. (1999) CapSelect: a highly sensitive method for 5' CAP-dependent enrichment of full length cDNA in PCR-mediated analyses of mRNAs. Nucleic Acid Res. 27, e31. 15. , Zhu, Y. , and Siebert, P. D. (1998) Gene Cloning and Analysis by RT-PCR (Siebert, P. ) BioTechniques, Natick, MA, 305–319.

1-kb BamHI-EcoRI insert bearing the P. freudenreichii cobA gene. The ClaI and BstEII sites lie outside the cobA gene. The sites within cobA were predicted from the sequence (Genbank accession no. U13043). ) cobA/cysGA Red Fluorescent Indicators 21 21 Fig. 3. The biosynthetic pathway from δ-ALA to two fluorescent porphyrinoid compounds, factor II and trimethylpyrrocorphin. ) 22 Roessner accumulate at relatively high levels, probably because of loss of feedback inhibition of aminolevulinic acid (ALA) synthesis in cells overexpressing urogen III methyltransferase (5) and stability of the products.

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