Hormone Action Part D by Nathan P. Kaplan, Nathan P. Colowick, Joel G. Hardman, Bert

By Nathan P. Kaplan, Nathan P. Colowick, Joel G. Hardman, Bert W. O'Malley

The significantly acclaimed laboratory ordinary, tools in Enzymology, is among the so much hugely revered guides within the box of biochemistry. due to the fact 1955, every one quantity has been eagerly awaited, usually consulted, and praised via researchers and reviewers alike. The sequence includes a lot fabric nonetheless proper this present day - really a necessary booklet for researchers in all fields of existence sciences.

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4] ~ H E PERFUSED RAT LIVER 35 I n most instances, perfusion with nonrecirculating medium is preferred. With this method, substrates and hormones can be maintained at constant physiological concentrations, and rapid changes in substrate utilization or product formation can be readily measured. Complications due to the transformation of substrates or products in the medium itself are also avoided with this method because substrates can be infused into the influent medium just prior to the portal cannulas and effluent medium can be immediately deproteinized.

Exton and C. R. Park, J. Biol. Chem. 242, 2622 (1967). 4 F. Assimacopoulos-Jeannet, J. H. Exton, and B. Jeanrenaud, Amer. J. Physiol. 225, 25 (1973). 26 MVEa [4] various components of the perfusion system proper to be dismantled (Fig. 1). ) of which is placed near the livers. Each of the two oxygenation chambers (Fig. 2) consists of a Plexiglas cylindrical drum (9 cm internal diameter), the external face of which is removable and allows penetration of a Teflon-sealed metal shaft carrying four stainless steel tubes for influent and effluent perfusion media and gases (Figs.

For most purposes, however, 200-300 g rats were used. If smaller animals are used, it may be necessary to decrease the size of the cannulas that are described below. 5 rag) 1 hour before use. ). The abdominal cavity was opened by making a transverse incision with the scissors just below the rib cage. The diaphragm was transected, and lateral incisions were made along both sides of the rib cage. The heart was exposed by folding back the cut section of the anterior chest wall. Prior to excising the heart, it was picked up with the fingers and the lungs were pushed toward the back.

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