By Nelson James Dunford, Jacob T. Schwartz, William G. Bade, Robert G. Bartle
Pt. 1. normal theory.--pt. 2. Spectral theory.--pt. three. Spectral operators
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Cut a disk of sequencing membrane to fit the internal diameter of the zerodead volume cartridge (for the Knauer cartridge, 6-mm diameter). 2. Wet the membrane with methanol and water. Insert the membrane disk into the cartridge and retain it in place with the aid of the Teflon O-ring. 3. 1 % aqueous TFA) and to the detector set at 215 nm. 4. 1 % TFA through the cartridge to obtain a stable baseline. 5. 2 mLimin. 6. 1 % TFA for about 10 mins. 7. Remove the membrane from the cartridge and store at 4°C until sequencing.
With the latter, these flow cells can be directly installed into diode-array detectors (DAD) with performance levels of signal to noise ratios close to standard flow cells (Moritz et ai, manuscript in preparation). OS-mm LD. D. fused-silica tubing. This tubing, which replaced the standard stainless steel electro spray needle, extended to the tip of the electrospray needle assembly. L. 44 CASTELLANOS-SERRA et al. 3 Applications to Proteome Analysis by Mass Spectrometry Identification and characterization of proteins isolated from natural sources by polyacrylamide gel electrophoresis has become a routine technique.
Trends BioI. Sci. 21, 494-495. Moertz E, Sareneva T, Haebel S, Julkunen I, Roepstorff P (1996) Mass spectrometric characterization of glycosylated interferon-g variants separated by gel electrophoresis. Electrophoresis 17: 925-931. Neubauer G and Mann M (1999) Mapping phosphorylation sites of gel-isolated proteins by nanoelectrospray: Potentials and limitations. Anal Chern. 71: 235 - 242. Oda Y, Chait BT (1998) Purification and identification of phosphopeptides. Proc. 46th ASMS Conference on Mass Spectrometry and Allied Topics Orlando, Florida, May 31-June 4 Olsen EHN, Rahbek-Nielsen H, Th0gersen IB, Roepstorff P, Enghild JJ (1998) Post-translational Modifications of Human Inter-a-Inhibitor: Identification of Glycans and Disulfide Bridges in Heavy Chains 1 and 2.