Microarrays: Volume 1: Synthesis Methods by Hartmut Seliger (auth.), Jang B. Rampal (eds.)

By Hartmut Seliger (auth.), Jang B. Rampal (eds.)

Microarray expertise, Volumes 1 and a pair of, current info in designing and fabricating arrays and binding experiences with organic analytes whereas supplying the reader with a large description of microarray expertise instruments and their strength functions. the 1st quantity offers with tools and protocols for the training of microarrays. the second one quantity info functions and information research, that is vital in studying the large info popping out of microarray experiments.

Among the subjects mentioned in quantity 1: Synthesis equipment, are matrices within the synthesis of microarrays, array optimization procedures, array-based comparative genomic hybridization, 60-mer oligonucleotide probes, bifunctional reagents NTMTA and NTPAC, and excessive density arrays utilizing electronic microarray synthesis systems. different subject matters comprise multiplex ligation-dependent probe amplification (MLPA), hybridization stipulations in situ-synthesized oligo arrays, peptide arrays, excessive density replication instruments (HDRT), protocols for the quantification of oligo hybridization, glyco-bead arrays, and an research into the rising nano technology.

Microarray expertise, Volumes 1 and a couple of, supply considerable info to all degrees of scientists from amateur to these in detail conversant in array technology.

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37. 38. 39. 40. 41. 42. 43. Seliger Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA. Nucl. Acids Res. 9, 879–894. Letsinger, R. L. and Lunsford, W. B. (1976) Synthesis of thymidine oligonucleotides by phosphite triester intermediates. J. Am. Chem. Soc. 98, 3656–3661. Caruthers, M. H. (1985) Gene synthesis machines: DNA chemistry and its uses. Science 230, 281–285. , Rossi, J. , and Wallace, R. B. (1984) Synthesis and the use of synthetic oligonucleotides. Annu. Rev.

Furthermore, the cells could be retrieved from the microchambers for analysis 20 Seliger of antibody-specific DNA. The studies described in ref. 210 use antibodies to proteins displayed on the cell surface to monitor, in high-throughput format, specific binding to different cell types applying appropriate fluorescent-labeled detection molecules (210). The monitoring of RNA interference-mediated loss of function was the topic of another publication (212). A considerable amount of research was triggered by a seminal paper by Ziauddin (213).

21, 1192–1199. 21. Khorana, H. G. (1979) Total synthesis of a gene. Science 203, 614–625. 22. Merrifield, R. B. (1963) Solid-phase peptide synthesis. I. The synthesis of a tetrapeptide. J. Am. Chem. Soc. 85, 2149–2154. 23. Letsinger, R. L. and Kornet, M. J. (1963) Popcorn polymer as a support in multistep synthesis. J. Am. Chem. Soc. 85, 3045–3046. 24. Letsinger, R. L. and Mahadevan, V. (1965) Oligonucleotide synthesis on a polymer support. J. Am. Chem. Soc. 87, 3526–3527. 25. Astell, C. R. and Smith, M.

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