Micropatterning in Cell Biology Part B by Matthieu Piel and Manuel Théry (Eds.)

By Matthieu Piel and Manuel Théry (Eds.)

This new quantity of equipment in mobilephone Biology is the second one quantity describing micropatterning, complementing quantity 119. Chapters are written by means of specialists within the box and comprise state of the art material.

  • Includes sections on micropatterning in second with photomask, maskless micropatterning and 2nd nanopatterning
  • Chapters are written by means of specialists within the field
  • Cutting-edge material

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A minimal midzone protein module controls formation and length of antiparallel microtubule overlaps. Cell, 142, 420–432. , & Mitchison, T. J. (1997). Microtubule polymerization dynamics. Annual Review of Cell and Developmental Biology, 13, 83–117. , Corral, M. , et al. (2009). Chromatin shapes the mitotic spindle. Cell, 138, 502–513. , & Surrey, T. (2013). Microtubule organization in vitro. Current Opinion in Cell Biology, 25(1), 23–29. , & Kirschner, M. (1985). Influence of the centrosome on the structure of nucleated microtubules.

Wilson, C. , Tsuchida, M. , Allen, G. , Barnhart, E. , Applegate, K. , Yam, P. , et al. (2010). Myosin II contributes to cell-scale actin network treadmilling through network disassembly. Nature, 465, 373–377. , Babcock, H. , & Zhuang, X. (2012). Dual-objective STORM reveals threedimensional filament organization in the actin cytoskeleton. Nature Methods, 9, 185–188. , & Svitkina, T. (2007). Novel roles of formin mDia2 in lamellipodia and filopodia formation in motile cells. PLoS Biology, 5, 317.

Proceed without delay to actin polymerization and observation. , 2012), and probe to what extent entanglement between adjacent branched networks is stable (Fig. 4). For such purpose the simplest geometry consists of two sets of parallel bars. The first set contains bars spaced apart with a variable distance and coated with NPFs. While the second surface-patterning set of bars, interdigitated with the first ones, is coated with myosins. NPFs-coated bars generate a dense actin network from which escape actin filaments oriented with their barbed ends growing outward and organized into a parallel actin structure.

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