Molecular Biology of RNA. New Perspectives by Masayori Inouye

By Masayori Inouye

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T h e s u b s t r a t e 3 ' - p h o s p h a t e is transferred to the 3'-terminal guanosine of the e n z y m e . T h e p H d e p e n d e n c e of the reaction (optimum at p H 5) indicates that the e n z y m e has activity t o w a r d the dianion and m u c h greater activity t o w a r d the m o n o a n i o n form of the 3'-phosphate of the substrate. Phosphorylation of the e n z y m e is reversible by C - O H and other oligo(pyrimidines) such as U C U - O H . T h u s , the R N A e n z y m e acts as a p h o s p h o t r a n s f e r a s e , transferring the 3'-terminal p h o s p h a t e of C p to U C U - O H with multiple t u r n o v e r (Fig.

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