Molecular biology problem solver : a laboratory guide by Alan S. Gerstein

By Alan S. Gerstein

Such a lot learn within the lifestyles sciences contains a middle set of molecular-based apparatus and techniques, for which there's no scarcity of step by step protocols. still, there continues to be a really excessive variety of inquiries put to advertisement technical help teams, particularly relating to difficulties.

Molecular Biology challenge Solver: A Laboratory Guide asks the reader to contemplate an important questions, such as:

  • Have you chose the main acceptable study strategy?
  • Have you pointed out the problems severe on your profitable program of a technique?
  • Are you accustomed to the restrictions of a given technique?
  • When may still universal procedural ideas of thumb now not be applied?
  • What ideas may well you observe to solve a problem?

A designated question-based layout reports universal assumptions and laboratory practices, with the purpose of providing a company realizing of ways suggestions and tactics paintings, in addition to how you can steer clear of difficulties. a few significant concerns explored by way of the book's professional participants include:

  • Working correctly with organic samples and radioactive materials
  • DNA and RNA purification
  • PCR
  • Protein and nucleid acid hybridization
  • Prokaryotic and eukaryotic expression systems
  • Properly utilizing and protecting laboratory equipment

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How Does the Meter Measure the Sample pH? . . . . What Is the Purpose of Autobuffer Recognition? . . . . Which Buffers Are Appropriate for Your Calibration Step? . . . . . . . . . . . . . . . . . . . . . . What Is Temperature Compensation and How Does One Choose the Best Method for an Analysis? . . . . How Does Resolution Affect pH Measurement? . . . . Why Does the Meter Indicate “Ready” Even as the pH Value Changes? . . . .

Practice . . . . . . . . . . . . . . . . . . . . . . Centrifugation of DNA and RNA . . . . . . . . . . . Troubleshooting . . . . . . . . . . . . . . . . . . . Pipettors . . . . . . . . . . . . . . . . . . . . . . . 51 51 51 54 54 55 55 55 58 63 64 67 Data on the performance characteristics of different protein concentration assays were generously provided by Bio Rad Inc. 49 Which Pipette Is Most Appropriate for Your Application?

How Does a Buffer Control the pH of a Solution? . . . When Is a Buffer Not a Buffer? . . . . . . . . . . . . What Are the Criteria to Consider When Selecting a Buffer? . . . . . . . . . . . . . . . . . . . . . What Can Generate an Incorrect or Unreliable Buffer? . . . . . . . . . . . . . . . . . . . . . What Is the Storage Lifetime of a Buffer? . . . . . . . 32 32 32 32 33 33 35 37 Editor’s note: Many, perhaps most, molecular biology procedures don’t require perfection in the handling of reagents and solution preparation.

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