Molecular Mechanisms of Oxygen Activation by Osamu Hayaishi

By Osamu Hayaishi

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Furthermore, llL-hydroxy-12,14eicosadienoic acid is formed from 11,14-eicosadienoic acid (35). Another monohydroxy acid formed from 8,11,14-eicosatrienoic acid is 12-hydroxy8,10-heptadecadienoic acid ( 3 4 - 3 6 ) . It could be concluded from isotope experiments that the three carbon atoms lost in the formation of this acid were not derived from the carboxyl end or the pentyl side chain of 8 , 1 1 , 1 4 eicosatrienoic acid. The compound was devoid of tritium when biosynthe3 3 3 sized from [ 9 - H ] - , [ 1 0 - H ] - , and [11- H]8,11,14-eicosatrienoic acids, indi­ cating that C-9 to C - l l of the precursor was eliminated in its formation (36).

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Chem. 2 4 6 , 7826-7829. 48. , and Hayaishi, O. (1968). Biochem. Biophys. Res. Commun. 3 2 , 30-36. 49. White-Stevens, R. , and Kamin, H. (1970). Biochem. Biophys. Res. Commun. 3 8 , 882-889. 50. , Hori, K , and Hayaishi, O. (1972). / . Biol. Chem. 2 4 7 , 3439-3444. 51. , and Hayaishi, O. (1972). Proc. Nat. Acad. Set. S. 69, 3723-3726. 52. Olson, J. , and Hayaishi, O. (1965). Proc. Nat. Acad. Sci. S. 5 4 , 1364-1370. 53. , and Hayaishi, O. (1965). Fed. } Fed. Amer. Soc. Exp. Biol. 2 4 , 604. 54.

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