By Thomas Wartmann, Gotthard Kunze (auth.), Prof. Dr. Klaus Wolf, Prof. Dr. Karin Breunig, Prof. Dr. Gerold Barth (eds.)
Most info on yeasts derives from experiments with the traditional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the total nuclear and mitochondrial genome of which has additionally been sequenced. For all different non-conventional yeasts, investigations are in growth and the fast improvement of molecular strategies has allowed an perception additionally right into a number of non-conventional yeasts.
In this bench handbook, over 70 useful protocols utilizing 15 diversified non-conventional yeast species and likewise numerous protocols of normal use are defined intimately. All of those experiments at the genetics, biochemistry and biotechnology of yeasts were contributed via well known laboratories and feature been reproduced time and again. The trustworthy protocols are hence perfect additionally for undergraduate and graduate functional courses.
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Extra info for Non-Conventional Yeasts in Genetics, Biochemistry and Biotechnology: Practical Protocols
Because of a certain toxicity work under a hood and avoid inhaling this substance. If greater amounts of the solvent have to be evaporated use diethyl ether free from peroxides. Check the diethyl ether with commercially available paper strips for peroxides and remove the peroxides if present by chemical methods. 7 Production of Aromatic Acids During the Oxidation of Phenylalkanes by Candida maltosa Experimental Procedure Cultivation of cells For cultivation of cells and induction of hydrocarbon-oxidizing enzymes use ten 500-mL Erlenmeyer flasks, filled with 100 mL of mineral salts medium, 1 mL of biotin solution and 1 % (v/v) n-dodecane.
These n-alkane-assimilating Candida yeasts are of interest as their metabolic intermediates can be used for industrial application such as perfume synthesis (Uemura 1985). Taxonomic classification of the genus Candida is usually based on cellular morphology and distinctive reactions in a standardized set of fermentation and assimilation tests (Meyer et al. 1998). However, these phenotypic characteristics are often strain-specific and inadequate for the recognition of species (Kurtzman and Robnett 1995).
Sp. 1098 (this study), C. sp. M1210 (this study), C. maltosa (U45745), C. viswanathii (U45752), C. lodderae (U45755), C. tropicalis pk233 (this study), C. albicans (U45776) and C. dubliniensis (U57685). The bar under the tree indicates the distance corresponding to five changes per 100 nucleotide positions References Arie M, Matsuda H, Furuhashi K, Takagi M (2000) Phylogenetic identification of n-alkane assimilating Candida yeasts based on nucleotide divergence in the 5¢ end of LSU rDNA gene. J Gen Appl Microbiol 46:257–262 Fukazawa Y, Nakase T, Shinoda T, Nishikawa A, Kagaya K, Tsuchiya T (1975) Significance of cell wall structures on yeast classification: proton magnetic resonance and serological and deoxyribonucleic acid characterization of Candida sake and related species.