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BLs are about 500 nm in diameter, making them smaller than Sonazoid which is US contrast agent (about 2 mm) (Figure 3(B)). We also conﬁrmed the structures of BLs by transmission electron microscopy and observed nanobubbles in the lipid bilayer (Figure 3(C)). Another morphological studies of BLs using transmission electron microscopy by Kodama et al. (2010) showed that gas and liquid seemed to be encapsulated together by a single lipid bilayer. 4. IN VITRO pDNA DELIVERY WITH BLs Gene therapy requires highly efﬁcient and safe gene delivery vectors.
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Physical methods of nucleic acid transfer: general concepts and applications. British Journal of Pharmacology, 157, 207–219. Vincent, K. , Shyu, K. , Tio, R. , et al. (2000). Angiogenesis is induced in a rabbit model of hindlimb ischemia by naked DNA encoding an HIF-1alpha/VP16 hybrid transcription factor. Circulation, 102, 2255–2261. , & Hua, Z. C. (2013). Systemic administration of attenuated in combination with interleukin-21 for cancer therapy. Molecular and Clinical Oncology, 1, 461–465. , & Lu, S.