Oxygen Transport to Tissue. Satellite Symposium of the 28th by A. G. B. Kovách, E. Dóra and M. Kessler (Eds.)

By A. G. B. Kovách, E. Dóra and M. Kessler (Eds.)

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Extra info for Oxygen Transport to Tissue. Satellite Symposium of the 28th International Congress of Physiological Sciences, Budapest, Hungary, 1980

Sample text

Thermal Transcoronary Transport Functions at Varied Myocardial Blood Flows. 0. At low flows there is earlier escape and a high peak compared to curves at high flows, indicating diffusional shunting. 3» Modelling of a Diffusional Bypass A mathematical model was composed of an array of parallel capillarytissue units with axial and transverse diffusion. The flows were all identical, but the array could be folded in a U-shape so that the inflow was close to the outflow region, so permitting a variable degree of shunting.

Scattering and absorption are investigated at each probe. Evaluation according to Lambert-Beer's law is applicable only to clear solutions. It is well known that scattering deforms the spectra. In the following an evaluation method for reflection spectra of biological material will be described that considers absorption, inhomogeneities and scattering according to the two-flux-theory after Kubelka and Munk (1931). Quantitative analysis of the spectra is performed by two steps: 1. calculation of true absorption from measured spectra and 2.

G. B. Kovach, E. Dora, M. Kessler, I. A. Silver (eds) INTRACELLULAR MICRO HETEROGENEITY OF OXYGEN CONCENTRATIONS I. S . L o n g m u i r , J . A . K n o p p , D . B e n s o n a n d H . , in press). This was demonstrated by staining non-respiring cells and tissues with pyrenebutyric (PBA) whose fluorescence is proportionately quenched by oxygen. V. microscopy in the absence and the presence of oxygen. Using the Stern-Volmer relationship on each pixel, the solubility coefficient for oxygen could be calculated.

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